SMAD4 Knockout cell line (Hep G2)

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SMAD4 Knockout cell line (Hep G2) Order

SMAD4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1526
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SMAD4 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SMAD4 Knockout cell line (Hep G2)
Species
Human
Gene Name
SMAD4
Gene ID
4089
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SMAD4-201)
Validation: PCR+Sanger Sequencing
SMAD4 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SMAD4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SMAD4 gene knockout SMAD4 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SMAD4 Knockout cell line (Hep G2)
SMAD4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1526
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SMAD4 Knockout cell line (THP-1)

    SMAD4 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HT0640
    YKO-HT0640(THP-1)
  • SMAD4 Knockout cell line (HCT 116)

    SMAD4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-H822
    YKO-H822(HCT 116)
  • SMAD4 Knockout cell line (HeLa)

    SMAD4 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HS3521
    YKO-HS3521(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SMAD4 Knockout cell line (THP-1)

    SMAD4 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HT0640
      inquiry   More details
  • SMAD4 Knockout cell line (HCT 116)

    SMAD4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-H822
      inquiry   More details
  • SMAD4 Knockout cell line (HeLa)

    SMAD4 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HS3521
      inquiry   More details

SMAD4 Knockout cell line (Hep G2) Order

SMAD4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1526
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SMAD4 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SMAD4 Knockout cell line (Hep G2)
Species
Human
Gene Name
SMAD4
Gene ID
4089
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SMAD4-201)
Validation: PCR+Sanger Sequencing
SMAD4 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SMAD4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SMAD4 gene knockout SMAD4 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SMAD4 Knockout cell line (Hep G2)
SMAD4 Knockout cell line (Hep G2)
Catalog#
YKO-HT1526
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SMAD4 Knockout cell line (THP-1)

    SMAD4 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HT0640
    YKO-HT0640(THP-1)
  • SMAD4 Knockout cell line (HCT 116)

    SMAD4 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-H822
    YKO-H822(HCT 116)
  • SMAD4 Knockout cell line (HeLa)

    SMAD4 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HS3521
    YKO-HS3521(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SMAD4 Knockout cell line (THP-1)

    SMAD4 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HT0640
      inquiry   More details
  • SMAD4 Knockout cell line (HCT 116)

    SMAD4 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-H822
      inquiry   More details
  • SMAD4 Knockout cell line (HeLa)

    SMAD4 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SMAD4
    Gene ID: 4089
    Size: 1*10^6
    Catalog#: YKO-HS3521
      inquiry   More details

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